Immunological changes in human skeletal muscle and blood after eccentric exercise and multiple biopsies

Citation
C. Malm et al., Immunological changes in human skeletal muscle and blood after eccentric exercise and multiple biopsies, J PHYSL LON, 529(1), 2000, pp. 243-262
Citations number
50
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Physiology
Journal title
JOURNAL OF PHYSIOLOGY-LONDON
ISSN journal
0022-3751 → ACNP
Volume
529
Issue
1
Year of publication
2000
Pages
243 - 262
Database
ISI
SICI code
0022-3751(20001115)529:1<243:ICIHSM>2.0.ZU;2-D
Abstract
1.. A role of the immune system in muscular adaptation to physical exercise has been suggested but data from controlled human studies are scarce. The present study investigated immunological events in human blood and skeletal muscle by immunohistochemistry and flow cytometry after eccentric cycling exercise and multiple biopsies. 2. Immunohistochemical detection of neutrophil- (CD11b, CD15), macrophage- (CD163), satellite cell- (CD56) and IL-1 beta -specific antigens increased similarly in human skeletal muscle after eccentric cycling exercise togethe r with multiple muscle biopsies, or multiple biopsies only. 3. Changes in immunological variables in blood and muscle were related, and monocytes and natural killer (NK) cells appeared to have governing functio ns over immunological events in human skeletal muscle. 4. Delayed onset muscle soreness, serum creatine kinase activity and C-reac tive protein concentration were not related to leukocyte infiltration in hu man skeletal muscle. 5. Eccentric cycling and/or muscle biopsies did not result in T cell infilt ration in human skeletal muscle. Modes of stress other than eccentric cycli ng should therefore be evaluated as a myositis model in human. 6. Based on results from the present study, and in the light of previously published data, it appears plausible that muscular adaptation to physical e xercise occurs without preceding muscle inflammation. Nevertheless, leukocy tes seem important for repair, regeneration and adaptation of human skeleta l muscle.