Identification of genes highly expressed in G2-arrested Chinese hamster ovary cells by differential display analysis

Citation
Y. Sasaki et al., Identification of genes highly expressed in G2-arrested Chinese hamster ovary cells by differential display analysis, J CL LAB AN, 14(6), 2000, pp. 314-319
Citations number
27
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Medical Research Diagnosis & Treatment
Journal title
JOURNAL OF CLINICAL LABORATORY ANALYSIS
ISSN journal
0887-8013 → ACNP
Volume
14
Issue
6
Year of publication
2000
Pages
314 - 319
Database
ISI
SICI code
0887-8013(2000)14:6<314:IOGHEI>2.0.ZU;2-S
Abstract
Abnormal cell cycle regulation is believed to be an important step in tumor igenesis. In mammalian cells, DNA damage commonly leads to cell cycle arres t in G2; however, little is known about the detailed biochemical mechanisms underlying the DNA damage-induced G2 arrest. In order to identify genes di fferentially expressed in association with G2 arrest, differential display analysis was performed between exponentially growing Chinese hamster ovary (CHO) cells and G2-arrested CHO cells induced by etoposide, SN-38, or X-rad iation. We identified five cDNA clones whose expression was upregulated in G2-arrested CHO cells. Sequence analysis revealed that three clones were ho mologous to known genes: isogene I of translation initiation factor eIF-4A, ribosomal protein L13, and translation repressor NAT1. The remaining two c lones showed no homology to known genes. These results indicate that DNA da mage can alter the expression of multiple genes, including translational re gulators. J. Clin. Lab. Anal. 14: 314-319, 2000. (C) 2000 Wiley-Liss, Inc.