Exaggerated vascular and renal pathology in endothelin-B receptor-deficient rats with deoxycorticosterone acetate-salt hypertension

Citation
Y. Matsumura et al., Exaggerated vascular and renal pathology in endothelin-B receptor-deficient rats with deoxycorticosterone acetate-salt hypertension, CIRCULATION, 102(22), 2000, pp. 2765-2773
Citations number
34
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cardiovascular & Respiratory Systems","Cardiovascular & Hematology Research
Journal title
CIRCULATION
ISSN journal
0009-7322 → ACNP
Volume
102
Issue
22
Year of publication
2000
Pages
2765 - 2773
Database
ISI
SICI code
0009-7322(20001128)102:22<2765:EVARPI>2.0.ZU;2-H
Abstract
Background-Endothelin (ET)-1 plays an important role in the pathogenesis of deoxycorticosterone acetate (DOCA)-salt-induced hypertension. We evaluated the pathological role of ETB receptors in DOCA-salt-induced hypertension, cardiovascular hypertrophy, and renal damage by using the spotting-lethal ( sl) rat, which carries a naturally occurring deletion in the ETB receptor g ene. Methods and Results-Homozygous (sl/sl) rats exhibit abnormal development of neural crest-derived epidermal melanocytes and the enteric nervous system, and they do not live beyond 1 month because of intestinal aganglionosis an d intestinal obstruction. The dopamine beta -hydroxylase (D betaH) promoter was used to direct ETB transgene expression in sl/sl rats to support norma l enteric nervous system development. D betaH-ETB sl/sl rats live into adul thood and are healthy, expressing ETB receptors in adrenal glands and other adrenergic neurons. When homozygous (sl/sl) and wild-type (+/+) rats, all of which were transgenic, were treated with DOCA-salt, homozygous rats exhi bited earlier and higher increases in systolic blood pressure than did wild -type rats. Chronic treatment with ABT-627, an ETA receptor antagonist, com pletely suppressed DOCA-salt-induced hypertension in both groups. Renal dys function and histological damage were more severe in homozygous than in wil d-type rats. Marked vascular hypertrophy was observed in homozygous rats th an in wild-type rats. Renal and vascular injuries were significantly improv ed by ABT-627. In DOCA-salt-treated homozygous rats, there were notable inc reases in renal, urinary, and aortic ET-1, all of which were normalized by ABT-627. Conclusions-ETB-mediated actions are protective in the pathogenesis of DOCA -salt-induced hypertension. Enhanced ET-1 production and ETA-mediated actio ns are responsible for the increased susceptibility to DOCA-salt hypertensi on and tissue injuries in ETB receptor-deficient rats.