Morphological and molecular evidence of the confusion between Elodea callitrichoides and E-nuttallii in Belgium and Northern France

Citation
A. Vanderpoorten et al., Morphological and molecular evidence of the confusion between Elodea callitrichoides and E-nuttallii in Belgium and Northern France, BELG J BOT, 133(1-2), 2000, pp. 41-52
Citations number
28
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences
Journal title
BELGIAN JOURNAL OF BOTANY
ISSN journal
0778-4031 → ACNP
Volume
133
Issue
1-2
Year of publication
2000
Pages
41 - 52
Database
ISI
SICI code
0778-4031(2000)133:1-2<41:MAMEOT>2.0.ZU;2-E
Abstract
The floristic data concerning Elodea callitrichoides in Belgium and Norther n France were checked. E. callitrichoides is easily distinguished from E. n uttalllii when flowering, however flowering plants have never been observed in the study area. Vegetatively, the leaves of E. callitrichoides are alwa ys plane and usually longer than those of E. nuttallii. On this basis, cert ain collections formerly determined as E. callitrichoides belong morphologi cally to E. nuttallii. E. nuttallii however displays such a polymorphism, t hat certain plants with plane leaves can hardly be definitely named. On the same plant, shoots or shoot segments with short recurved leaves and others with long plane or twisted leaves can be observed. Molecular markers were used to determine dubious populations from Northern France exhibiting long plane or twisted leaves by comparing their Amplified Fragments Length Polym orphism (AFLP) to that of other populations of E. nuttallii, genuine flower ed E. callitrichoides from Alsace and E. canadensis. The two couple of prim ers Eco-AGG/Mse-CAT and Eco-ACA/Mse-CAG provided 103 scorable fragments. Th ese allow the determination of sterile Elodea. Based on these morphological and genetic data, the populations formerly referred as E. callitrichoides from Northern France belong to E. nuttallii. E. callitrichoides should be d eleted from the regional flora. The determination keys should emphasize the difficulties to distinguish vegetatively plants with long plane leaves. Th e investigated populations of E. nuttallii exhibited a high level of geneti c polymorphism which needs further investigations in relationship with its cytological and morphological variability and the possible introgression wi th E. canadensis.