Infectious hypodermal and hematopoietic necrosis virus of shrimp is related to mosquito Brevidensoviruses

Citation
H. Shike et al., Infectious hypodermal and hematopoietic necrosis virus of shrimp is related to mosquito Brevidensoviruses, VIROLOGY, 277(1), 2000, pp. 167-177
Citations number
48
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
VIROLOGY
ISSN journal
0042-6822 → ACNP
Volume
277
Issue
1
Year of publication
2000
Pages
167 - 177
Database
ISI
SICI code
0042-6822(20001110)277:1<167:IHAHNV>2.0.ZU;2-W
Abstract
We purified and sequenced infectious hypodermal and hematopoietic necrosis virus (IHHNV), a small DNA virus of shrimp, from wild Penaeus stylirostris. The virion has a buoyant density of 1.45 as determined by cesium chloride gradient. Analysis of 3873 nucleotides of the viral genome revealed three l arge open reading frames (ORFs) and parts of the noncoding termini of the v iral genome. The left, mid, and right ORFs on the complementary (plus) stra nd have potential coding capacities of 666 amino acids (aa) (75.77 kDa), 36 3 aa (42.11 kDa), and 329 aa (37.48 kDa), respectively. The overall genomic organization is similar to that of the mosquito brevidensoviruses. The lef t ORF most likely encodes the major nonstructural (NS) protein (NS-1) since it contains conserved replication initiator motifs and NTP-binding and hel icase domains similar to those in NS-1 from all other parvoviruses. The IHH NV putative NS-1 shares the highest aa sequence homology with the NS-1 of m osquito brevidensoviruses, Aedes densovirus and Aedes albopictus parvovirus . A search for putative splicing sites revealed that the N-terminal region of NS-1 is very likely located in a small ORF upstream of the left ORF. The right ORF is presumed to encode structural polypeptides (VPs), as in other parvoviruses. Two putative promoters, located upstream of the left and rig ht ORFs, are presumed to regulate expression of NS and VP genes, respective ly. Thus, IHHNV is closely related to densoviruses of the genus Brevidensov irus in the family Parvoviridae, and we therefore propose to rename this vi rus Penaeus stylirostris densovirus(PstDNV). (C) 2000 Academic Press.