A chemical genomics approach toward understanding the global functions of the target of rapamycin protein (TOR)

Citation
Tf. Chan et al., A chemical genomics approach toward understanding the global functions of the target of rapamycin protein (TOR), P NAS US, 97(24), 2000, pp. 13227-13232
Citations number
53
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Multidisciplinary
Journal title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN journal
0027-8424 → ACNP
Volume
97
Issue
24
Year of publication
2000
Pages
13227 - 13232
Database
ISI
SICI code
0027-8424(20001121)97:24<13227:ACGATU>2.0.ZU;2-L
Abstract
The target of rapamycin protein (TOR) is a highly conserved ataxia telangie ctasia-related protein kinase essential for cell growth. Emerging evidence indicates that TOR signaling is highly complex and is involved in a variety of cellular processes. To understand its general functions, we took a chem ical genomics approach to explore the genetic interaction between TOR and o ther yeast genes on a genomic scale. In this study, the rapamycin sensitivi ty of individual deletion mutants generated by the Saccharomyces Genome Del etion Project was systematically measured. Our results provide a global vie w of the rapamycin-sensitive functions of TOR. In contrast to conventional genetic analysis, this approach offers a simple and thorough analysis of ge netic interaction on a genomic scale and measures genetic interaction at di fferent possible levels. It can be used to study the functions of other dru g targets and to identify novel protein components of a conserved core biol ogical process such as DNA damage checkpoint/repair that is interfered with by a cell-permeable chemical compound.