Identification of a novel human BCL-X promoter and exon

Citation
L. Maccarthy-morrogh et al., Identification of a novel human BCL-X promoter and exon, ONCOGENE, 19(48), 2000, pp. 5534-5538
Citations number
19
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
ONCOGENE
ISSN journal
0950-9232 → ACNP
Volume
19
Issue
48
Year of publication
2000
Pages
5534 - 5538
Database
ISI
SICI code
0950-9232(20001116)19:48<5534:IOANHB>2.0.ZU;2-7
Abstract
BCL-X-L is a key anti-apoptotic BCL-2 family protein that is widely express ed in human cancer cells and is induced in response to diverse survival sig nals. The translation initiation codon for BCL-X-L is located in BCL-X exon II and previous analyses have indicated that BCL-X-L RNAs initiate close t o the start of exon IT or additionally contain a non-coding first exon (exo n IA) spliced to exon TI, Using 5' RACE we have now identified a novel BCL- X non-coding exon (exon IB) which is spliced directly to exon IJ[ in place of exon IA, Exon IB-containing RNAs encoded BCL-X-L and were detected in no n-malignant lymphocytes and lymphoma cells from lymph node biopsies and mer e expressed at significant levels in cell lines derived from ovarian, colon and breast cancers. We identified two TATA-hox sequences upstream of exon TB and demonstrated that surrounding genomic sequences contained strong pro moter activity in lymphoma cells (approximately 300-fold active relative to controls). We have therefore identified a powerful new BCL-X promoter and a novel exon that contributes to BCL-X-L expression.