Translin binds to the sequences adjacent to the breakpoints of the TLS andCHOP genes in liposarcomas with translocation t(12;16)

Citation
T. Hosaka et al., Translin binds to the sequences adjacent to the breakpoints of the TLS andCHOP genes in liposarcomas with translocation t(12;16), ONCOGENE, 19(50), 2000, pp. 5821-5825
Citations number
18
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
ONCOGENE
ISSN journal
0950-9232 → ACNP
Volume
19
Issue
50
Year of publication
2000
Pages
5821 - 5825
Database
ISI
SICI code
0950-9232(20001123)19:50<5821:TBTTSA>2.0.ZU;2-O
Abstract
Myxoid and round-cell liposarcomas share the translocation t(12;16)(q13;p11 ) creating the TLS-CHOP fusion gene as a common genetic alteration. We prev iously reported several unique characteristics of genomic sequences around the breakpoints in the TLS and CHOP loci, and among them was the presence o f consensus recognition motifs of Translin, a protein that associates with chromosomal translocations of lymphoid neoplasms. We further extended our s earch for Translin binding motifs in sequences adjacent to breakpoints and investigated,whether Translin binds to these sequences in vitro by mobility -shift assay. Computer-assisted search found sequences highly homologous (> 70%) with Translin binding motifs adjacent to the breakpoints in 10 out of 11 liposarcomas,vith the TLS-CHOP fusion genes. All of 13 oligonucleotides corresponding to the putative binding sequences in these cases bind to Hela cell extract and also recombinant Translin protein, although the binding a ffinity of each motif showed considerable differences. The DNA-protein comp lex formation was inhibited by non-labeled competitor or anti-Translin anti body, suggesting the specificity of the complex formation. Considering the high incidence and specific binding property, the presence of Translin bind ing motif may be one of the important determinants for the location of brea kpoints in the TLS and CHOP gents in liposarcomas.