Expression of foreign genes in primary cultured cells of the blue shrimp Penaeus stylirostris

Citation
H. Shike et al., Expression of foreign genes in primary cultured cells of the blue shrimp Penaeus stylirostris, MARINE BIOL, 137(4), 2000, pp. 605-611
Citations number
26
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Aquatic Sciences
Journal title
MARINE BIOLOGY
ISSN journal
0025-3162 → ACNP
Volume
137
Issue
4
Year of publication
2000
Pages
605 - 611
Database
ISI
SICI code
0025-3162(200011)137:4<605:EOFGIP>2.0.ZU;2-D
Abstract
The ability to introduce and express foreign genes can greatly facilitate i n vitro studies of the molecular biology of an organism. We report here the first use of modified retroviral vectors to achieve foreign gene expressio n in primary cultured cells from the Oka organ and ovary of the blue shrimp Penaeus stylirostris (Litopenaeus) stylirostris (Holthuis 1980). Of the he terologous promoters tested, the Moloney murine leukemia virus and Rous sar coma virus promoters mediated the highest levels of reporter gene expressio n. Firefly luciferase and Escherichia coli beta -galactosidase were success fully used as reporter genes in cultured cells from both Oka organ and ovar y. These in vitro systems can be exploited for the study of shrimp cellular biology and regulation of gene expression.