Determination of L-iminoethyl-L-lysine in serum by liquid chromatography

Citation
U. Werner et al., Determination of L-iminoethyl-L-lysine in serum by liquid chromatography, J CHROMAT B, 749(1), 2000, pp. 35-40
Citations number
12
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis
Journal title
JOURNAL OF CHROMATOGRAPHY B
ISSN journal
1387-2273 → ACNP
Volume
749
Issue
1
Year of publication
2000
Pages
35 - 40
Database
ISI
SICI code
1387-2273(20001110)749:1<35:DOLISB>2.0.ZU;2-N
Abstract
A selective and sensitive method is presented for the determination of L-im inoethyl-L-lysine (L-NIL) in rat serum. L-NIL is a selective inhibitor of t he inducible nitric oxide synthase. The analytical technique is based upon a two-buffer reversed-phase HPLC system with fluorescence detection of pre- column derivatized amino acid analogue with o-phtaldialdehyde. The retentio n time of L-NIL was 19.4 min. The limit of quantification was 0.5 mg/l. Aft er validation, the method was used to study the pharmacokinetic profile of L-NIL in rats after intravenous as well as oral administration of a single dose. (C) 2000 Elsevier Science B.V. All rights reserved.