BRCA1 physically and functionally interacts with ATF1

Citation
Y. Houvras et al., BRCA1 physically and functionally interacts with ATF1, J BIOL CHEM, 275(46), 2000, pp. 36230-36237
Citations number
62
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF BIOLOGICAL CHEMISTRY
ISSN journal
0021-9258 → ACNP
Volume
275
Issue
46
Year of publication
2000
Pages
36230 - 36237
Database
ISI
SICI code
0021-9258(20001117)275:46<36230:BPAFIW>2.0.ZU;2-F
Abstract
BRCA1, a breast and ovarian cancer susceptibility gene, encodes a 220-kDa p rotein whose precise biochemical function::remains unclear. BRCA1 contains an N-terminal- RING finger that mediates protein-protein interaction. The C -terminal domain of BRCA1 (BRCT) can activate transcription and interacts w ith RNA polymerase holoenzyme. Using the yeast two-hybrid system, we identi fied an interaction between the BRCA1 RING finger and ATF1, a member of the cAMP response element-binding protein/activating transcription factor (CRE B/ATF) family. We demonstrate that BRCA1 and ATF1 can physically associate in vitro, in yeast, and in human cells. BRCA1 stimulated transcription from a cAMP response-element reporter gene in transient transfections. BRCA1 al so stimulated transcription from a natural promoter, that of tumor necrosis factor-alpha, in a manner dependent on the integrity of the cAMP response element. These results implicate BRCA1 in transcriptional activation of ATF 1 target genes, some of which are involved in the transcriptional response to DNA damage.