Multicolor spectral karyotype analysis of a transplantable human ileal carcinoid

Citation
H. Sjogren et al., Multicolor spectral karyotype analysis of a transplantable human ileal carcinoid, INT J MOL M, 6(6), 2000, pp. 629-633
Citations number
13
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Medical Research General Topics
Journal title
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
ISSN journal
1107-3756 → ACNP
Volume
6
Issue
6
Year of publication
2000
Pages
629 - 633
Database
ISI
SICI code
1107-3756(200012)6:6<629:MSKAOA>2.0.ZU;2-E
Abstract
In this report we present the results of a combined cytogenetic and multico lor spectral karyotype (SKY) analysis of a transplantable human ileal carci noid (GOT1). By using SKY it was possible to identify the origin and organi zation of all clonal marker chromosomes and to identify cryptic translocati ons not detectable by conventional chromosome banding. The stemline karyoty pe of low passage GOT1 cells was interpreted as 43,XX, der(1)del(1)(?), inv (2)(p25q13), del(3)(p21), del(5)(q13q31), del(6)(q13), -9, -13, -15, del(16 ) (q22). Analysis of the GOT1 cells after about 2.5 years of propagation in nude mice allowed us to follow the in vivo progression of this tumor. Rela tively few additional rearrangements had occurred during this period, indic ating that the GOT1 cells are genetically stable. Most of the abnormalities detected result in loss of whole or parts of chromosomes, suggesting that loss of multiple chromosomal regions, presumably containing tumor suppresso r genes, might be important genetic events in ileal carcinoids.