Liquid extraction and HPLC-DAD assay of hydrochlorothiazide from plasma for a bioequivalence study at the lowest therapeutic dose

Citation
A. Medvedovici et al., Liquid extraction and HPLC-DAD assay of hydrochlorothiazide from plasma for a bioequivalence study at the lowest therapeutic dose, EUR J DRUG, 25(2), 2000, pp. 91-96
Citations number
22
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Pharmacology & Toxicology
Journal title
EUROPEAN JOURNAL OF DRUG METABOLISM AND PHARMACOKINETICS
ISSN journal
0378-7966 → ACNP
Volume
25
Issue
2
Year of publication
2000
Pages
91 - 96
Database
ISI
SICI code
0378-7966(200004/06)25:2<91:LEAHAO>2.0.ZU;2-Q
Abstract
The main parameters considered in optimizing the liquid extraction and quan titative assay were the yield, precision, limit of quantification, time req uired for extraction and concentration, and quantity of solvent. The influe nce on these parameters of the following factors was examined: nature of th e extracting solvent, quantity of solvent, co-extraction solvent, and durat ion of stirring. instead of equilibrium parameters of the involved thermody namic system, a kinetic approach was preferred in terms of the effective pa rtition 'constant', which is not really constant but a function of time and extraction conditions. The final selected method, considered to be rapid a nd simple, was applied to determine the pharmacokinetics of hydrochlorotiaz ide (HCT) after administration of Capozide (Bristol-Myers Squibb) tablets c ontaining 50 mg Captopril and 25 mg HCT, to 4 healthy volunteers. The resul ts obtained were in accordance with the pharmacokinetic parameters of HCT r eported in the literature.