Chemotactic activity for polymorphonuclear leukocytes: Meconium versus meconium-stained amniotic fluid

Citation
T. Yamada et al., Chemotactic activity for polymorphonuclear leukocytes: Meconium versus meconium-stained amniotic fluid, AM J REPROD, 44(5), 2000, pp. 275-278
Citations number
20
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
ISSN journal
1046-7408 → ACNP
Volume
44
Issue
5
Year of publication
2000
Pages
275 - 278
Database
ISI
SICI code
1046-7408(200011)44:5<275:CAFPLM>2.0.ZU;2-#
Abstract
PROBLEM: Our previous study indicated that meconium-stained amniotic fluid (turbid AF) possessed a potent chemotactic activity for leukocytes, which m ay be dependent on interleukin-8. It is not known, however, whether meconiu m itself possesses this chemotactic activity. METHOD OF STUDY: Meconium samples were collected from mature neonates with and without turbid AF. A 5% meconium suspension in phosphate buffered salin e was prepared and measured for its chemotactic activity for leukocytes usi ng the blind well chamber technique. Concentrations of IL-8, TNF alpha and IL-1 beta were also measured with ELISA. RESULTS: The number of leukocytes that migrated to the meconium suspension (35 +/- 27) was comparable with that of the clear AF (31 +/- 37), but was s ignificantly lower than that of the turbid AF (184 +/- 62, P < 0.0001). The meconium suspension contained much lower levels of IL-8, TNF<alpha> and IL -1 beta than the turbid AF. CONCLUSIONS: Meconium itself exhibits a lower chemotactic activity for poly morphonuclear leukocytes than turbid AF in vitro. The leukocyte chemotactic activity of turbid AF does not originate from meconium itself.