Crystallization and preliminary X-ray analysis of endopolygalacturonase SE1 from Trichosporon penicillatum

Authors
Lu, CT Tada, T Nakamura, Y Wada, K Nishimura, K Katsuya, Y Sawada, M Takao, M Sakai, T
Citation
Ct. Lu et al., Crystallization and preliminary X-ray analysis of endopolygalacturonase SE1 from Trichosporon penicillatum, ACT CRYST D, 56, 2000, pp. 1668-1669
Citations number
11
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis
Journal title
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
ISSN journal
0907-4449 → ACNP
Volume
56
Year of publication
2000
Part
12
Pages
1668 - 1669
Database
ISI
SICI code
0907-4449(200012)56:<1668:CAPXAO>2.0.ZU;2-Z
Abstract
Endopolygalacturonase SE1 from Trichosporon penicillatum has been crystalli zed by the hanging-drop method of vapour diffusion using ammonium sulfate a s a precipitant. The crystals belong to the hexagonal space group P6(1) or P6(5), with unit-cell parameters a = b = 135.0, c = 70.7 Angstrom, gamma = 120 degrees. The calculated V-M based on one molecule per asymmetric unit i s 3.09 Angstrom (3) Da(-1). A native data set from a crystal has been colle cted to 2.0 Angstrom resolution on a Cu K alpha rotating-anode X-ray source .