The mucinous layer of corneal endothelial cells

Citation
Ek. Kim et al., The mucinous layer of corneal endothelial cells, YONSEI MED, 41(5), 2000, pp. 651-656
Citations number
6
Language
INGLESE
art.tipo
Article
Categorie Soggetti
General & Internal Medicine
Journal title
YONSEI MEDICAL JOURNAL
ISSN journal
0513-5796 → ACNP
Volume
41
Issue
5
Year of publication
2000
Pages
651 - 656
Database
ISI
SICI code
0513-5796(200010)41:5<651:TMLOCE>2.0.ZU;2-X
Abstract
Purpose: Thc goal of this study was to characterize the morphology of the m ucinous layer on rabbit, bovine, owl, and human corneal endothelial cells. Methods: Corneoscleral buttons were fixed using cetylpyridinium chloride to stabilize "mucus" and the tissue was prepared for transmission electron mi croscopy. Photomicrographs were measured to determine chi thickness of thc endothelial and epithelial mucinous layer in the central cornea. Results: T he endothelial mucinous layer was seen as a nearly uniform electrodense reg ion on the apical aspect of the endothelium. it was found to be 0.9 mum, 0. 9 mum, 0.9 mum, anti 0.5 mum thick in rabbit, bovine, owl, and human, respe ctively. The owl endothelium had an additional lass electrodense layer with a granular appearance and a thickness of about 200 mum. The mucinous layer on the epithelium was similar in appearance to that on the endothelium and across species. Conclusions: The morphologic similarity of the endothelial and epithelial mucinous layers is a serendipitous finding that should prov e valuable in experimental design. Ultimately, it is hoped that studies of the posterior corneal surface will deepen our knowledge of endothelial prot ection.