Genomic analysis and functional expression of canine dopamine D2 receptor

Citation
H. Myeong et al., Genomic analysis and functional expression of canine dopamine D2 receptor, GENE, 257(1), 2000, pp. 99-107
Citations number
30
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
GENE
ISSN journal
0378-1119 → ACNP
Volume
257
Issue
1
Year of publication
2000
Pages
99 - 107
Database
ISI
SICI code
0378-1119(20001017)257:1<99:GAAFEO>2.0.ZU;2-I
Abstract
Dopamine D2 receptor (DRD2) is one of the five dopamine receptors with seve n transmembrane domains that are coupled to the G protein. We have cloned a nd characterized the genomic and cDNA sequences of the canine DRD2 gene, wh ich are 12.7 and 2.7 kb in size, respectively. The genomic DNA is composed of seven exons and six introns, encoding a 443 amino acid protein with 95% amino acid identity to other mammalian D2 receptors. A length polymorphism was detected in intron 3 of the receptor gene. We also characterized altern atively spliced forms of DRD2 cDNAs, DRD2L and DRD2S. They showed a higher level of expression in midbrain and thalamus. The ratio between the long an d short form is similar in RT-PCR reaction. In human and rodent, the same t wo spliced forms are known to be coupled to G(i)-type heterotrimeric GTP bi nding protein, thereby opening an inwardly rectifying potassium channel, GI RK1. When the canine DRD2L and DRD2S were heterologously expressed in Xenop us oocytes, both forms activated GIRK1 potassium channels through coupling with Gi protein. This activation was dose-dependent, demonstrating its liga nd specificity. (C) 2000 Elsevier Science B.V. All rights reserved.