Function of microtubules in intercellular transport of plant virus RNA

Citation
V. Boyko et al., Function of microtubules in intercellular transport of plant virus RNA, NAT CELL BI, 2(11), 2000, pp. 826-832
Citations number
50
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell & Developmental Biology
Journal title
NATURE CELL BIOLOGY
ISSN journal
1465-7392 → ACNP
Volume
2
Issue
11
Year of publication
2000
Pages
826 - 832
Database
ISI
SICI code
1465-7392(200011)2:11<826:FOMIIT>2.0.ZU;2-5
Abstract
Cell-to-cell progression of tobacco mosaic virus (TMV) infection in plants depends on virus-encoded movement protein (MP). Here we show that a conserv ed sequence motif in tobamovirus MPs shares similarity with a region in tub ulins that is proposed to mediate lateral contacts between microtubule prot ofilaments. Point mutations in this motif confer temperature sensitivity to microtubule association and viral-RNA intercellular-transport functions of the protein, indicating that MP-interacting microtubules are functionally involved in the transport of vRNA to plasmodesmata. Moreover, we show that MP interacts with microtubule-nucleation sites. Together, our results indic ate that MP may mimic tubulin assembly surfaces to propel vRNA transport by a dynamic process that is driven by microtubule polymerization.