cAMP-GEFII is a direct target of cAMP in regulated exocytosis

Citation
N. Ozaki et al., cAMP-GEFII is a direct target of cAMP in regulated exocytosis, NAT CELL BI, 2(11), 2000, pp. 805-811
Citations number
43
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell & Developmental Biology
Journal title
NATURE CELL BIOLOGY
ISSN journal
1465-7392 → ACNP
Volume
2
Issue
11
Year of publication
2000
Pages
805 - 811
Database
ISI
SICI code
1465-7392(200011)2:11<805:CIADTO>2.0.ZU;2-R
Abstract
Although cAMP is well known to regulate exocytosis in many secretory cells, its direct target in the exocytotic machinery is not known. Here we show t hat cAMP-GEFII, a cAMP sensor, binds to Rim (Rab3-interacting molecule, Rab 3 being a small G protein) and to a new isoform, RimP, both of which are pu tative regulators of fusion of vesicles to the plasma membrane. We also sho w that cAMP-GEFII, through its interaction with Rim2, mediates cAMP-induced , Ca2+-dependent secretion that is not blocked by an inhibitor of cAMP-depe ndent protein kinase (PKA). Accordingly, cAMP-GEFII is a direct target of c AMP in regulated exocytosis and is responsible for cAMP-dependent, PKA-inde pendent exocytosis.