CELLULAR-LOCALIZATION OF THROMBOPOIETIN MESSENGER-RNA IN THE LIVER BYIN-SITU HYBRIDIZATION

Citation
S. Nomura et al., CELLULAR-LOCALIZATION OF THROMBOPOIETIN MESSENGER-RNA IN THE LIVER BYIN-SITU HYBRIDIZATION, Experimental hematology, 25(7), 1997, pp. 565-572
Citations number
41
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Medicine, Research & Experimental",Hematology
Journal title
ISSN journal
0301-472X
Volume
25
Issue
7
Year of publication
1997
Pages
565 - 572
Database
ISI
SICI code
0301-472X(1997)25:7<565:COTMIT>2.0.ZU;2-E
Abstract
The expression of thrombopoietin (TPO) mRNA is observed in several tis sues, including liver, kidney, brain, skeletal muscle, intestine, sple en, and bone marrow. Among these organs, the highest expression of TPO mRNA is detected in the liver. We identified cells producing TPO by m eans of in situ hybridization of adult rat liver using digoxigenin-11- UTP-labeled cRNA probes. We found that the cells expressing TPO mRNA a lso expressed serum albumin mRNA. TPO mRNA was detected in parenchymal cells (hepatocytes) but not in non-parenchymal cells (including endot helial cells, epithelial cells, and so forth). To determine the locati on of TPO expression in embryogenesis, sections of fetal mice were fur ther analyzed by in situ hybridization. TPO mRNA was detected only in hepatocytes of fetal liver, which was also the major site of hematopoi esis. The expression of TPO mRNA in fetal liver was observed from 12.5 days post-coitus. Northern blot analysis showed that mouse liver tran scribed the same size of TPO mRNA in the fetus and in the adult. These results clearly demonstrate that hepatocytes are the primary site of TPO production in the liver from fetus to adult.