Suppressors of transforming growth factor-beta pathway mutants in the Caenorhabditis elegans dauer formation pathway

Citation
T. Inoue et Jh. Thomas, Suppressors of transforming growth factor-beta pathway mutants in the Caenorhabditis elegans dauer formation pathway, GENETICS, 156(3), 2000, pp. 1035-1046
Citations number
37
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology,"Molecular Biology & Genetics
Journal title
GENETICS
ISSN journal
0016-6731 → ACNP
Volume
156
Issue
3
Year of publication
2000
Pages
1035 - 1046
Database
ISI
SICI code
0016-6731(200011)156:3<1035:SOTGFP>2.0.ZU;2-F
Abstract
The dauer is a developmentally arrested alternative third larval stage of C aenorhabditis elegans. Entry into this state is regulated by environmental cues, including temperature, food, and the concentration of constitutively secreted dauer pheromone. Genetically, three parallel pathways have been fo und that regulate this process. Of these, the group 2 pathway, which includ es die genes daf-1, daf-3, daf-4, daf-5, daf-7: daf-8, and dnf-14, mediates the transduction of environmental signals through the ASI chemosensory neu ron and encodes a TGF-P-related signaling pathway. To identify additional g enes that function in this pathway, we carried out a screen for suppressors of mutations in daf-l, daf-8, and daf-14. From die total of 36 mutations, seven complementation groups were identified. Three complementation groups correspond to the previously described genes daf-3, daf-5, and daf-12. Thre e correspond to novel genes scd-l, scd-2 and scd-3. Genetic analysis of the se scd genes is presented here. A fourth complementation group was represen ted by a single mutation sa315, which affects the daf-2/age-1 insulin-relat ed signaling pathway.