Differential regulation of amoA and amoB gene copies in Nitrosomonas europaea

Citation
Ly. Stein et al., Differential regulation of amoA and amoB gene copies in Nitrosomonas europaea, FEMS MICROB, 192(2), 2000, pp. 163-168
Citations number
16
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
FEMS MICROBIOLOGY LETTERS
ISSN journal
0378-1097 → ACNP
Volume
192
Issue
2
Year of publication
2000
Pages
163 - 168
Database
ISI
SICI code
0378-1097(20001115)192:2<163:DROAAA>2.0.ZU;2-3
Abstract
Nitrosomonas europaea contains two nearly identical copies of the operon, a moCAB, which encodes the ammonia monooxygenase (AMO) enzyme. Cells of N. eu ropaea containing single mutations in either amoA or amoB gene copies were incubated in ammonium both prior to and after exposure to acetylene or ligh t. For each strain, the O-2 consumption rates and amounts of AmoA polypepti de, the active site-containing subunit of AMO, produced in each strain were determined. Strains carrying a mutation in either the amoA(2) or amoB(2) g enes responded similarly to wild-type cells, but the strains carrying mutat ions in the amoA(1) or amoB(1) genes responded differently from the wildtyp e, or from each other. These results suggest that the copies of amoA and am oB are differentially regulated upon exposure to different external stimuli . (C) 2000 Federation of European Microbiological Societies. Published by E lsevier Science B.V. All rights reserved.