Diabetes affects phospholipid content in the nuclei of the rat liver

Citation
M. Zendzian-piotrowska et al., Diabetes affects phospholipid content in the nuclei of the rat liver, HORMONE MET, 32(10), 2000, pp. 386-389
Citations number
28
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Endocrinology, Nutrition & Metabolism
Journal title
HORMONE AND METABOLIC RESEARCH
ISSN journal
0018-5043 → ACNP
Volume
32
Issue
10
Year of publication
2000
Pages
386 - 389
Database
ISI
SICI code
0018-5043(200010)32:10<386:DAPCIT>2.0.ZU;2-W
Abstract
The aim of the present study was to examine the effect of acute streptozoto cin diabetes on the content of different phospholipids and the incorporatio n of blood-borne C-14-palmitic acid into the phospholipid moieties in the r at liver nuclei. Diabetes was produced by intravenous administration of str eptozotocin, and determinations were carried out two and seven days thereaf ter. Phospholipids were extracted from isolated nuclei and separated into t he following fractions: sphingomyelin, phosphatidylcholine, phosphatidylser ine, phosphatidylinositol, phosphatidylethanolamine and cardiolipin. Follow ing that, they were quantified and radioactivity was measured. It was found that, in comparison to non-diabetic controls, two-day diabetes reduced the total content of phospholipids in the nuclei by 9.6%. The content of phosp hatidylcholine and phosphatidylserine was reduced and the content of the re maining phospholipids was stable. The specific activity of phosphatidylchol ine, phosphatidylserine, phosphatidylethanolamine and cardiolipin, based on radioactivity incorporated from C-14-palmitic acid, was elevated. Seven-da y diabetes resulted in a reduction of the total phospholipid content in the nuclei by 39.4 %. This was accounted for by a reduction in the content of each phospholipid fraction with the exception of cardiolipin. The specific activity of each phospholipid fraction, was elevated in this group. It is c oncluded that insulin is involved in the regulation of the nuclear phosphol ipid content.