Expression of acute and late-stage inflammatory antigens, c-fms, CSF-1, and human monocytic serine esterase 1, in tumor-associated macrophages of renal cell carcinomas

Citation
B. Hemmerlein et al., Expression of acute and late-stage inflammatory antigens, c-fms, CSF-1, and human monocytic serine esterase 1, in tumor-associated macrophages of renal cell carcinomas, CANCER IMMU, 49(9), 2000, pp. 485-492
Citations number
34
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
CANCER IMMUNOLOGY IMMUNOTHERAPY
ISSN journal
0340-7004 → ACNP
Volume
49
Issue
9
Year of publication
2000
Pages
485 - 492
Database
ISI
SICI code
0340-7004(200011)49:9<485:EOAALI>2.0.ZU;2-G
Abstract
Purpose: Tumor cells influence the differentiation of infiltrating macropha ges. In the present study, the differentiation of macrophages in renal cell carcinomas was investigated with special regard to their possible role ill tumor growth and spread. Methods: Macrophages were characterized by means of immunohistochemistry of the Ki-M1P, 25F9, MRP8, MRP14, and MRP8/14 antig ens and by means of in situ hybridization of CSF-1, its c-fins-coded corres ponding receptor, and human monocytic serine esterase-1 (HMSE-1) mRNA. Macr ophage subgroups were quantified within central tumor tissue, the correspon ding turner host interface, and tumor-free tissue and correlated with tumor necrosis, fibrosis, and tumor stage and grade. Results: Macrophage density was much higher within tumor tissue and the tumor/host interface than in t umor-free tissue. Well-differentiated carcinomas showed a lower degree of m acrophage density than less-differentiated carcinomas. Tumor-associated mac rophages could be divided into an active inflammatory typo (MR14(+), MRP8/1 4(+)) and into a late-phase inflammatory type (25F9(+), MRP8(+)). Necrosis was seen in less-differentiated carcinomas and associated with a significan tly increased density of MRP14(+) macrophages, which, however, did not corr elate with the extent of necrosis. The density of 25F9(+) macrophages was c orrelated with an extensive connective tissue formation and an advanced tum or stage. c-fms, CSF-I, and HMSE-1 mRNA expression did not discriminate bet ween the macrophage subgroups. Conclusions: Tumor-associated macrophages of the late-stage inflammatory type potentially support the spread of renal c ell cancer. CSF-1 derived from tumor cells and macrophages acts as a monocy te attractant and induces macrophage differentiation able to modulate the e xtracellular matrix rather than to exert cytotoxicity. CSF-1 derived from t umor cells and macrophages acts as a monocyte attractant and induces macrop hage differentiation able to modulate the extracellular matrix rather than to exert cytotoxicity.