Separation and determination of alantolactone and isoalantolactone in traditional Chinese herbs by capillary electrophoresis

Citation
Kt. Wang et al., Separation and determination of alantolactone and isoalantolactone in traditional Chinese herbs by capillary electrophoresis, TALANTA, 52(6), 2000, pp. 1001-1005
Citations number
18
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
TALANTA
ISSN journal
0039-9140 → ACNP
Volume
52
Issue
6
Year of publication
2000
Pages
1001 - 1005
Database
ISI
SICI code
0039-9140(20000905)52:6<1001:SADOAA>2.0.ZU;2-F
Abstract
A simple micellar electrokinetic chromatography (MEKC) method, using a 20 m M berate buffer (pH 8.0) with 25 mM SDS in the presence of 10% (V/V) methan ol, was established for the identification and determination of two isomers , alantolactone (AL) and isoalantolactone (IAL), in the extracts of Inula h elenium L. and Inula racemosa Hook f. Regression equations revealed linear relationships (correlation coefficients: 0.9990 for AL, 0.9991 for IAL) bet ween the peak area of AL and IAL and their concentration. The relative stan dard deviations of the migration time and peak area of the two constituents were 1.51, 1.62 and 2.01, 1.98%, respectively. The recoveries of the two c onstituents ranged between 95-105% for AL and 93-108% for IAL. (C) 2000 Els evier Science B.V. All rights reserved.