Non-linear calibration in quantitative analysis by HPTLC utilizing a fibreoptic fluorescence detector

Citation
R. Linares et al., Non-linear calibration in quantitative analysis by HPTLC utilizing a fibreoptic fluorescence detector, J LIQ CHR R, 23(17), 2000, pp. 2653-2668
Citations number
20
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN journal
1082-6076 → ACNP
Volume
23
Issue
17
Year of publication
2000
Pages
2653 - 2668
Database
ISI
SICI code
1082-6076(2000)23:17<2653:NCIQAB>2.0.ZU;2-1
Abstract
The non-linear calibration in quantitative determinations by high performan ce thin-layer chromatography utilizing a fibre optic fluorescence detector is examined. Dansyl derivatives of polyamines, as model compounds, are sepa rated in HPTLC silica-gel plates with chloroform:triethylamine (TEA):polyox yethylene-10-lauryl ether (POLE) (8:4:5%) (v:v:m) as solvent. The obtained calibration graphs show a non-linear relationship between the fluorescence intensity and the quantity of the amine (0-150ng). The study of the non-linearity shows that the best fits are obtained for th e quadratic and cubic regressions. However, for concentrations lower than 5 0 ng, linear calibrations are obtained with R-squared > 0.99. Furthermore, the principal parameters used to characterize the methods of analysis by HP TLC with fluorescence detection are calculated: sensibility, detection limi t, precision, and accuracy.