Mechanism of anti-tumor effect of combination of bleomycin and shock waves

Citation
M. Kato et al., Mechanism of anti-tumor effect of combination of bleomycin and shock waves, JPN J CANC, 91(10), 2000, pp. 1065-1072
Citations number
47
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
JAPANESE JOURNAL OF CANCER RESEARCH
ISSN journal
0910-5050 → ACNP
Volume
91
Issue
10
Year of publication
2000
Pages
1065 - 1072
Database
ISI
SICI code
0910-5050(200010)91:10<1065:MOAEOC>2.0.ZU;2-0
Abstract
We have previously reported marked enhancement of the cytocidal effect of b leomycin (BLM) on cancer cell suspensions in vitro by the combination with shock waves, In this study, we evaluated the synergistic effects on cancer cell proliferation and apoptosis in solid tumors. A spherical piezoceramic element was used as the shock wave source, with a pressure peak of 40 MPa, A human colon cancer cell line, SW480 was implanted onto the back of nude m ice. Two thousand shock waves were administered to the tumor immediately fo llowing an intravenous injection of BLM at a dose of one-tenth of the LD50, The tumor was extirpated at 3, 6, 12, 24, 72 h and 1 week following shock exposure. Cell proliferation and apoptosis were detected by Ki-67 using ant ibody MIB-1 and by the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick-end labeling (TUNEL) method. The lowest percentage (35.7% ) of Ki-67-positive cells appeared 24 h following the treatment. The maximu m apoptotic index was detected within 6 h following the treatment. Moreover , numerous large cells with enlarged nuclei were detected histologically, T hese results suggest that shock waves may enhance chemotherapeutic effects by increasing apoptosis and decreasing cell proliferation in the tumor tiss ue.