No significant correlation exists between core promoter mutations, viral replication, and liver damage in chronic hepatitis B infection

Citation
Yk. Chun et al., No significant correlation exists between core promoter mutations, viral replication, and liver damage in chronic hepatitis B infection, HEPATOLOGY, 32(5), 2000, pp. 1154-1162
Citations number
45
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Gastroenerology and Hepatology","da verificare
Journal title
HEPATOLOGY
ISSN journal
0270-9139 → ACNP
Volume
32
Issue
5
Year of publication
2000
Pages
1154 - 1162
Database
ISI
SICI code
0270-9139(200011)32:5<1154:NSCEBC>2.0.ZU;2-T
Abstract
Hepatitis B virus (HBV) core promoter mutants have been proposed to contrib ute to severe liver damage by increasing viral loads, but this has not yet been clearly shown. To examine the effects of core promoter mutants on vira l load and liver damage, we first developed a polymerase chain reaction (PC R)-based semiquantitative HBV DNA detection method with a high sensitivity (able to detect as low as 10(3) molecules/mL). Then we cloned 12 predominan t core promoter mutants from 41 chronic hepatitis B patients. The in vitro promoter and replication activities of these mutants were similar to those of wild-type virus. However, viral load was highly variable, and this was d ependent on individual patients rather than mutant type. In addition, there was no mutant type that showed any unique correlation with alanine transam inase (ALT) levels. Viral load was not significantly correlated with ALT le vel in both cross-sectional and longitudinal studies. Quantitation of HBV l evels also revealed no clear correlation between hepatitis B e antigen (HBe Ag) status and viral load. Taken together, these results indicated that the replication activity of core promoter mutants has little effect on viral l oad, and that viral load does not correlate with the severity of liver dama ge or with HBeAg status.