Gastric proton pump is expressed in the inner ear and choroid plexus of the rat

Citation
E. Lecain et al., Gastric proton pump is expressed in the inner ear and choroid plexus of the rat, HEARING RES, 149(1-2), 2000, pp. 147-154
Citations number
51
Language
INGLESE
art.tipo
Article
Categorie Soggetti
da verificare
Journal title
HEARING RESEARCH
ISSN journal
0378-5955 → ACNP
Volume
149
Issue
1-2
Year of publication
2000
Pages
147 - 154
Database
ISI
SICI code
0378-5955(200011)149:1-2<147:GPPIEI>2.0.ZU;2-Q
Abstract
Inner ear fluids and cerebrospinal fluid show remarkably stable ionic conce ntrations, particularly that of K+ and H+, but the mechanisms which control the homeostasis of these media are not well understood. We investigated a possible role of the gastric H,K-ATPase (gH,K-ATPase) pump in this control since this pump is known to be expressed in other tissues than gastric pari etal cells. Here, we show by reverse transcription-polymerase chain reactio n that the rat gH,K-ATPase alpha- and beta-subunits are expressed in the in ner ear (lateral wall, organ of Corti and spiral ganglion cells), while onl y the alpha-subunit is expressed in the choroid plexus (CP). The presence o f the alpha-subunit in the inner ear and CP was confirmed by immunoblotting . Immunohistochemistry localized this protein in the intermediate cells of the stria vascularis, in the spiral ligament and the spiral ganglion. gH,K- ATPase could be involved in the maintenance of H+ and K+ equilibria in cero brospinal and labyrinthine fluids. (C) 2000 Elsevier Science B.V. All right s reserved.