Column efficiency and separation of DNA fragments using slalom chromatography: Hydrodynamic study and fractal considerations

Citation
Yc. Guillaume et al., Column efficiency and separation of DNA fragments using slalom chromatography: Hydrodynamic study and fractal considerations, ANALYT CHEM, 72(20), 2000, pp. 4846-4852
Citations number
44
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
ANALYTICAL CHEMISTRY
ISSN journal
0003-2700 → ACNP
Volume
72
Issue
20
Year of publication
2000
Pages
4846 - 4852
Database
ISI
SICI code
0003-2700(20001015)72:20<4846:CEASOD>2.0.ZU;2-7
Abstract
Novel equations (Guillaume Y. C.; et al. Anal. Chem, 2000, 72, 853) were de veloped to describe the large double-stranded DNA molecule retention in sla lom chromatography (SC). These equations were applied for the first time to model both the "apparent selecticity" and the resolution between tao elute d DNA fragments on a chromatogram, a study of the column efficiency corrobo rated the fact that slalom chromatography is not based on an adsorption or equilibrium phenomenon, but can be attributed to a hydrodynamic phenomenon. Using a combination of the dynamics of DNA fragment progression in the col umn and fractal considerations, it was shown that the apparent selectivity depends both on the DNA fragment sizes and mobile-phase flow rate and there fore a balance between two hydrodynamic regimes. A chromatographic response function was also used to obtain the most efficient separation conditions for a mixture of DNA fragments in a minimum analysis time. The chromatograp hic data confirmed that in SC the now rate can increase or maintain the sep aration efficiency with an associated decrease in the analysis time. This c onstitutes an attractive outcome in relation to the classical chromatograph ic separation.