Y. Mishima et al., UTILIZATION OF AN OSMIUM COMPLEX AS A SEQUENCE RECOGNIZING MATERIAL FOR DNA-IMMOBILIZED ELECTROCHEMICAL SENSOR, Analytica chimica acta, 345(1-3), 1997, pp. 45-50
A novel immobilized DNA electrode based on a porous carbon electrode w
as prepared to study interactions between DNA and chemical compounds.
The characteristics of this electrode were evaluated by electrochemica
l methods, and then the interaction between DNA and an osmium complex
was studied. On this electrode (electrode surface; 0.66-2.37 cm(2)), D
NA was immobilized at 0.26x10(-4)-1.23x10(-4) mol cm(-2). Hybridizatio
n of DNA was observed with a relative standard deviation of 11% (n=4).
The tris(bipyridyl)osmium(II/III) ion (osmium complex) was found to i
nteract to major or minor grooves. Using this osmium complex, a DNA se
quence could be recognized with more than 12 mer of the same sequence.