UTILIZATION OF AN OSMIUM COMPLEX AS A SEQUENCE RECOGNIZING MATERIAL FOR DNA-IMMOBILIZED ELECTROCHEMICAL SENSOR

Citation
Y. Mishima et al., UTILIZATION OF AN OSMIUM COMPLEX AS A SEQUENCE RECOGNIZING MATERIAL FOR DNA-IMMOBILIZED ELECTROCHEMICAL SENSOR, Analytica chimica acta, 345(1-3), 1997, pp. 45-50
Citations number
16
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry Analytical
Journal title
ISSN journal
0003-2670
Volume
345
Issue
1-3
Year of publication
1997
Pages
45 - 50
Database
ISI
SICI code
0003-2670(1997)345:1-3<45:UOAOCA>2.0.ZU;2-C
Abstract
A novel immobilized DNA electrode based on a porous carbon electrode w as prepared to study interactions between DNA and chemical compounds. The characteristics of this electrode were evaluated by electrochemica l methods, and then the interaction between DNA and an osmium complex was studied. On this electrode (electrode surface; 0.66-2.37 cm(2)), D NA was immobilized at 0.26x10(-4)-1.23x10(-4) mol cm(-2). Hybridizatio n of DNA was observed with a relative standard deviation of 11% (n=4). The tris(bipyridyl)osmium(II/III) ion (osmium complex) was found to i nteract to major or minor grooves. Using this osmium complex, a DNA se quence could be recognized with more than 12 mer of the same sequence.