Overexpression of a gene encoding a cytochrome p450, CYP78A9, induces large and seedless fruit in arabidopsis

Citation
T. Ito et Em. Meyerowitz, Overexpression of a gene encoding a cytochrome p450, CYP78A9, induces large and seedless fruit in arabidopsis, PL CELL, 12(9), 2000, pp. 1541-1550
Citations number
43
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT CELL
ISSN journal
1040-4651 → ACNP
Volume
12
Issue
9
Year of publication
2000
Pages
1541 - 1550
Database
ISI
SICI code
1040-4651(200009)12:9<1541:OOAGEA>2.0.ZU;2-9
Abstract
An activation tagging screen in which the cauliflower mosaic virus 35S enha ncer was inserted randomly into an Arabidopsis genome homozygous for the fl oral homeotic mutation apetala2-1 (ap2-1) resulted in a line (28-5) with ex traordinarily wide, heart-shaped ovaries. The ovary of the 28-5 ap2-1 mutan t shows an oval shape because of increased numbers of enlarged cells. When the ap2-1 mutation is crossed out of the genetic background, more elongated rather than wider fruits are obtained. Normally, Arabidopsis fruits will d evelop to a normal size only when the ovules are present and fertilized. In the 28-5 single mutant, the siliques keep growing despite failure of ferti lization and can reach nearly normal size. When wild-type pollen was used t o pollinate the mutant pistil, the pollinated 28-5 silique became >10% long er and 40% wider than a wild-type silique, although producing very few seed s. The enhancer insertion in line 28-5 acts by hyperactivating a cytochrome P450 gene, CYP78A9. The pistil of 28-5 ap2-1 mutant flowers shows a struct ure similar to that of Capsella bursa-pastoris, a distant mustard relative of Arabidopsis, suggesting that the processes regulated by the CYP78A9-enco ded protein may be involved in evolutionary control of carpel shape.