Mutants of a temperature-sensitive two-P domain potassium channel

Citation
Mt. Kunkel et al., Mutants of a temperature-sensitive two-P domain potassium channel, J NEUROSC, 20(20), 2000, pp. 7517-7524
Citations number
37
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences & Behavoir
Journal title
JOURNAL OF NEUROSCIENCE
ISSN journal
0270-6474 → ACNP
Volume
20
Issue
20
Year of publication
2000
Pages
7517 - 7524
Database
ISI
SICI code
0270-6474(20001015)20:20<7517:MOATTD>2.0.ZU;2-E
Abstract
Within the Caenorhabditis elegans genome there exist at least 42 genes enco ding TWK (two-P domain K+) channels, potassium channel subunits that contai n two pore regions and four transmembrane domains. We now report the first functional characterization of a TWK channel from C. elegans. Although pota ssium channels have been reported to be activated by a variety of factors, TWK-18 currents increase dramatically with increases in temperature. Two mu tant alleles of the twk-18 gene confer uncoordinated movement and paralysis in C. elegans. Expression of wild-type and mutant TWK-18 channels in Xenop us oocytes showed that mutant channels express much larger potassium curren ts than wild-type channels. Promoter-green fluorescent protein fusion exper iments indicate that TWK-18 is expressed in body wall muscle. Our genetic a nd physiological data suggest that the movement defects observed in mutant twk-18 animals may be explained by an increased activity of the mutant TWK- 18 channels.