Apoptosis and its regulation in flat-type early colorectal carcinoma - Comparison with that in polypoid-type early colorectal carcinoma

Citation
A. Ozawa et al., Apoptosis and its regulation in flat-type early colorectal carcinoma - Comparison with that in polypoid-type early colorectal carcinoma, DIS COL REC, 43(10), 2000, pp. S23-S28
Citations number
27
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Gastroenerology and Hepatology
Journal title
DISEASES OF THE COLON & RECTUM
ISSN journal
0012-3706 → ACNP
Volume
43
Issue
10
Year of publication
2000
Supplement
S
Pages
S23 - S28
Database
ISI
SICI code
0012-3706(200010)43:10<S23:AAIRIF>2.0.ZU;2-D
Abstract
PURPOSE: The aim of this study was to investigate the relationship among ap optotic cell death, proliferative activity, and the expression of apoptosis -regulating proteins (p53, p21 (WAF1/CIP1), and bax) in flat-type early col orectal carcinoma and to compare these factors with those in polypoid-type early colorectal carcinoma. METHODS: Formalin-fixed, paraffin-embedded tiss ues of 11 flat-type early colorectal carcinomas and 17 polypoid-type early carcinomas were studied. The histologic diagnosis was either well-different iated adenocarcinoma or carcinoma in adenoma, and the depth of invasion was limited to mucosa or submucosa. Apoptotic cells were detected by terminal deoxynucleotide transferase-mediated deoxyuridine triphosphate-biotin nick end-labeling method, and proliferative activity was determined by Ki-67 imm unohistochemistry using monoclonal antibody MIB-1. Apoptosis-regulating pro teins were determined by immunohistochemistry using antibody DO-7 (p53), Ci p1 (p21 (WAF1/CIP1)), and Bar (bax). RESULTS: There was no significant diff erence in terminal deoxynucleotide transferase-mediated deoxyuridine tripho sphate-biotin nick end-labeling index between flat-type early colorectal ca rcinoma and polypoid-type early carcinoma, at 1.9 vs. 1.1, respectively. In flat-type carcinoma terminal deoxynucleotide transferase-mediated deoxyuti dine triphosphate-biotin nick end-labeling index in the p53 protein overexp ression group was significantly smaller than that in the p53 protein-negati ve group (P < 0.05). The Ki-67 labeling index/terminal deoxynucleotide tran sferase-mediated deoxyuridine triphosphate-biotin nick end-labeling index r atio in the p53 protein overexpression group was significantly higher than that in the p53 protein-negative group (P < 0.05). In polypoid-type carcino ma, the terminal deoxynucleotide transferase-mediated deoxyuridine triphosp hate-biotin nick end-labeling index and Ki67/ terminal deoxynucleotide tran sferase-mediated deoxyuridine triphosphate-biotin nick end-labeling index r atio showed no significant difference between the p53 protein overexpressio n group and p53 protein-negative group. CONCLUSION: p53-dependent apoptosis may contribute to the development of flat-type early colorectal carcinoma. Apoptosis and its regulation in flat-type early colorecral carcinoma may d iffer from those in polypoid-type carcinoma.