Molecular strategies for interrupting arthropod-borne virus transmission by mosquitoes

Citation
Cd. Blair et al., Molecular strategies for interrupting arthropod-borne virus transmission by mosquitoes, CLIN MICROB, 13(4), 2000, pp. 651
Citations number
95
Language
INGLESE
art.tipo
Review
Categorie Soggetti
Microbiology
Journal title
CLINICAL MICROBIOLOGY REVIEWS
ISSN journal
0893-8512 → ACNP
Volume
13
Issue
4
Year of publication
2000
Database
ISI
SICI code
0893-8512(200010)13:4<651:MSFIAV>2.0.ZU;2-9
Abstract
Arthropod-borne virus (arbovirus) infections cause a number of emerging and resurgent human and veterinary infectious diseases. Traditional means of c ontrolling arbovirus diseases include vaccination of susceptible vertebrate s and mosquito control, but in many cases these have been unavailable or in effective, and so novel strategies for disease control are needed. One poss ibility is genetic manipulation of mosquito vectors to render them unable t o transmit arboviruses. This review describes recent work to test the conce pt of pathogen-derived resistance in arthropods by expression of viral gene s in mosquito cell cultures and mosquitoes. Sense and antisense genome sequ ences fram La Crosse virus (LAC) (a member of the Bunyaviridae) and dengue viruses serotypes 1 to 4 (DEN-1 to DEN-4) (members of the Flaviviridae) wer e expressed in mosquito cells from double-subgenomic and replicon vectors b ased on Sindbis virus (a member of the Togaviridae). The cells were then ch allenged with homologous or related viruses. For LAG, expression of antisen se sequences front the small (S) genome segment, particularly full-length a ntisense S RNA, effectively interfered with replication of challenge virus, whereas expression of either antisense or sense RNA from the medium (M) se gment was completely ineffective in LAC inhibition. Expression of sense and antisense RNA derived from certain regions of the DEN genome also blocked homologous virus replication more effective than did RNA from other regions . Other parameters of RNA-mediated interference have been defined, such as the time when replication is blocked and the minimum size of effector RNA. The mechanism of RNA inhibition has not been determined although it resembl es double-stranded RNA interference in other nonvertebrate systems. Prospec ts for application of molecular strategies to control arbovirus diseases ar e briefly reviewed.