Multiplex PCR: Optimization and application in diagnostic virology

Citation
Em. Elnifro et al., Multiplex PCR: Optimization and application in diagnostic virology, CLIN MICROB, 13(4), 2000, pp. 559
Citations number
125
Language
INGLESE
art.tipo
Review
Categorie Soggetti
Microbiology
Journal title
CLINICAL MICROBIOLOGY REVIEWS
ISSN journal
0893-8512 → ACNP
Volume
13
Issue
4
Year of publication
2000
Database
ISI
SICI code
0893-8512(200010)13:4<559:MPOAAI>2.0.ZU;2-S
Abstract
PCR has revolutionized the field of infectious disease diagnosis. To overco me the inherent disadvantage of cost and to improve the diagnostic capacity of the test, multiplex PCR a variant of the test in which more than one ta rget sequence is amplified using more than one pair of primers, has been de veloped. Multiplex PCRs to detect viral bacterial and/or other infectious a gents in one reaction tube have been described Early studies highlighted th e obstacles that can jeopardize the production of sensitive and specific mu ltiplex assays, brit more recent studies have provided systematic protocols and technical improvements for simple test design. The most useful of thes e are the empirical choice of oligonucleotide primers and the use of hot st art-based PCR methodology. These advances along with others to enhance sens itivity and specificity and to facilitate automation have resulted in the a ppearance of numerous publications regarding the application of multiplex P CR in the diagnosis of infectious agents, especially those which target vir al nucleic acids. This article reviews the principles, optimization, and ap plication of multiplex PCR for the detection of viruses of clinical and epi demiological importance.