Advantages of using nested collision induced dissociation/post-source decay with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry: sequencing of novel peptides from wasp venom

Citation
M. Hisada et al., Advantages of using nested collision induced dissociation/post-source decay with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry: sequencing of novel peptides from wasp venom, RAP C MASS, 14(19), 2000, pp. 1828-1834
Citations number
17
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
ISSN journal
0951-4198 → ACNP
Volume
14
Issue
19
Year of publication
2000
Pages
1828 - 1834
Database
ISI
SICI code
0951-4198(2000)14:19<1828:AOUNCI>2.0.ZU;2-4
Abstract
We have examined the applicability of the 'nested' collision induced dissoc iation/post-source decay (CID/PSD) method to the sequencing of novel peptid es from solitary wasps which have neurotoxic venom for paralyzing other ins ects. The CID/PSD spectrum of a ladder peptide derived from an exopeptidase digest was compared with that of the intact peptide. The mass peaks observ ed only in the CID/PSD spectrum of a ladder peptide were extracted as C-ter minal fragment ions. Assignment of C-terminal fragment ions enabled calcula tion of N-terminal fragment masses, leading to differentiation between N-te rminal fragment ions and internal fragment ions. This methodology allowed r apid and sensitive identification by removing ambiguity in the assignment o f the fragment ions, and proved useful for sequencing unknown peptides, in particular those available as natural products with a limited supply. Copyr ight (C) 2000 John Wiley & Sons, Ltd.