In vitro plant regeneration in Macroptilium atropurpureum, a legume with abroad symbiont range for nodulation

Citation
H. Ezura et al., In vitro plant regeneration in Macroptilium atropurpureum, a legume with abroad symbiont range for nodulation, PLANT SCI, 159(1), 2000, pp. 21-27
Citations number
28
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT SCIENCE
ISSN journal
0168-9452 → ACNP
Volume
159
Issue
1
Year of publication
2000
Pages
21 - 27
Database
ISI
SICI code
0168-9452(20001008)159:1<21:IVPRIM>2.0.ZU;2-E
Abstract
Macroptilium atropurpureum is a model legume with a broad symbiont range fo r nodulation. We have achieved the first in vitro plant regeneration of thi s species using cv. Siratro. Hypocotyl explants excised from dark-grown see dlings generated slimy, friable calli after three weeks' culture on B5 medi um containing 1-2 mg/l kinetin and 0.05 mg/l alpha-naphthaleneacetic acid. This was followed by the generation of green organogenic callus with shoot buds by subculturing the explants to hormone-free B5 medium 20 days after t he start of culture. The green organogenic calli with shoot buds were maint ained as organogenic callus by subculturing on the same medium, and shoots were elongated on hormone-free B5 medium. Elongated shoots were rooted on h alf-strength B5 medium. Most regenerated plants were morphologically normal , diploid and fertile, although tetraploid plants appeared at a low frequen cy (8%). (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.