IMMUNOHISTOCHEMICAL STUDY ON GALACTOSAMINE-INDUCED SUBACUTE HEPATITISIN RATS OF JCL - WISTAR-TGN (ARGHGEN) 1NTS STRAIN (MINI RATS)

Citation
K. Uetsuka et al., IMMUNOHISTOCHEMICAL STUDY ON GALACTOSAMINE-INDUCED SUBACUTE HEPATITISIN RATS OF JCL - WISTAR-TGN (ARGHGEN) 1NTS STRAIN (MINI RATS), Experimental animals, 46(3), 1997, pp. 203-209
Citations number
23
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Veterinary Sciences",Zoology
Journal title
ISSN journal
1341-1357
Volume
46
Issue
3
Year of publication
1997
Pages
203 - 209
Database
ISI
SICI code
1341-1357(1997)46:3<203:ISOGSH>2.0.ZU;2-G
Abstract
Immunohistochemical study was carried out on D-galactosamine hydrochlo ride (GalN)-induced subacute hepatitis in rats of JCL:Wistar-TGN (ARGH GEN) 1Nts strain (Mini rats), in which the expression of growth hormon e gene is suppressed by the presence of an antisense transgene. Mini r ats were given 1000 mg/kg of GalN once a week for 4 consecutive weeks and killed at 1, 2, 3 and 4 weeks after the first administration. At I week after the first administration, proliferation of small epithelia l cells positive for both crfetoprotein and cytokeratin 7, i.e. so-cal led oval cells, was observed in the whole area of each hepatic lobule, and prominent deposition of fibronectin, laminin and type IV collagen was detected around these oval cells. Together with these extracellul ar matrix components, many activated Ito cells positive for both desmi n and alpha-smooth muscle actin were obsereved With time, most of the oval cells formed duct-like structures and lost their positive stainab ility for alpha-fetoprotein, and many Ito cells became inactive. Depos ition of fibronectin decreased rapidly from 2 weeks after the first ad ministration. At 4 weeks after the first administration, deposition of laminin was detected only around the duct-like structures, where that of type IV collagen was also still prominent. These results suggest t hat a large population of oval cells differentiated into bile duct epi thelial cells and that Ito cells and extracellular matrix components m ight play a role in this process.