Choice of spectroscopic lineshape model affects metabolite peak areas and area ratios

Citation
I. Marshall et al., Choice of spectroscopic lineshape model affects metabolite peak areas and area ratios, MAGN RES M, 44(4), 2000, pp. 646-649
Citations number
10
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Radiology ,Nuclear Medicine & Imaging","Medical Research Diagnosis & Treatment
Journal title
MAGNETIC RESONANCE IN MEDICINE
ISSN journal
0740-3194 → ACNP
Volume
44
Issue
4
Year of publication
2000
Pages
646 - 649
Database
ISI
SICI code
0740-3194(200010)44:4<646:COSLMA>2.0.ZU;2-L
Abstract
The use of Lorentzian model lineshapes leads to systematic errors in the qu antification of in vivo H-1 NMR spectra, Experimental lineshapes are better modeled by the Voigt (mixed Lorentzian-Gaussian) function, leading to more accurate fits (reduced chi(2)). In this work, results from a group of 41 s ubjects are presented. It is shown that not only are the estimated metaboli te peak areas affected by the choice of lineshape model, but so too are the metabolite ratios. For example, the NAA/choline ratio was 1.92 +/- 0.06 (m ean +/- standard error) using the Lorentzian lineshape model and 1.85 +/- 0 .05 using the Voigt lineshape model. The corresponding figures for NAA/crea tine were 2.32 +/- 0.06 and 2.10 +/- 0.05 respectively, which are significa ntly different for the two lineshape models, An explanation of this previou sly unreported effect is given, This finding clearly has serious implicatio ns for the methodology and reporting of spectroscopic studies. (C) 2000 Wil ey-Liss, Inc.