Induction of cytokine genes and IL-1 alpha by chemical hypoxia in PC12 cells

Citation
Mt. Liu et al., Induction of cytokine genes and IL-1 alpha by chemical hypoxia in PC12 cells, LIFE SCI, 67(18), 2000, pp. 2147-2157
Citations number
35
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
LIFE SCIENCES
ISSN journal
0024-3205 → ACNP
Volume
67
Issue
18
Year of publication
2000
Pages
2147 - 2157
Database
ISI
SICI code
0024-3205(20000922)67:18<2147:IOCGAI>2.0.ZU;2-2
Abstract
The role of cytokine in neuronal injury was examined in rat pheochromocytom a (PC12) cells under chemical hypoxia (i.e. KCN) and glucose deprivation. T he mRNA levels of interleukin-1 alpha (IL-1 alpha), IL-6, and tumor necrosi s factor-alpha (TNF-alpha) were measured by reverse transcription-polymeras e chain reaction (RT-PCR) in PC12 cells exposed to 0.5 mn KCN for various t ime intervals. Cytokine mRNA levels expressed to peak levels 30 minutes aft er KCN treatment and declined gradually until 240 min. The IL-1 alpha activ ity reached the highest levels 2 hr after the same KCN treatment. Under par allel conditions, KCN increased cytosolic free calcium concentration ([Ca2](i)) in the absence of glucose. However, IL-1 alpha rnRNA induction by KCN was not altered under calcium-free conditions in PC12 cells, indicating it s induction was Ca2+-independent. However, the phosphatidylcholine (PC)spec ific phospholipase C (PLC) inhibitor D609 decreased the KCN-induced IL-1 al pha mRNA and protein in PC12 cells suggests that PC-PLC might play a role i n cytokine induction during hypoxia. (C) 2000 Elsevier Science Inc. All rig hts reserved.