Effect of triiodothyronine on the content of phospholipids in the rat liver nuclei

Citation
R. Bucki et al., Effect of triiodothyronine on the content of phospholipids in the rat liver nuclei, J PHYSL PH, 51(3), 2000, pp. 535-540
Citations number
26
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Physiology
Journal title
JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY
ISSN journal
0867-5910 → ACNP
Volume
51
Issue
3
Year of publication
2000
Pages
535 - 540
Database
ISI
SICI code
0867-5910(200009)51:3<535:EOTOTC>2.0.ZU;2-1
Abstract
The aim of the present study was to examine the effect of triiodothyronine (T-3) on the content of phospholipids and on the incorporation of blood-bor ne palmitic acid into the phospholipid moieties in the nuclei of the rat li ver. T-3 was administered daily for 7 days, 10 mu g x 100 g(-1). The contro l rats were treated with saline. Each rat received C-14-palmitic acid, intr avenously suspended in serum. 30 min after administration of the label, sam ples of the liver were taken. The nuclei were isolated in sucrose gradient. Phospholipids were extracted from the nuclei fraction and from the liver h omogenate. They were separated into the following fractions: sphingomyelin, phosphatidylcholine, phosphatidylserine, phosphatidylinositol, phosphatidy lethanolamine and cardiolipin. The content and radioactivity of each fracti on was measured. It was found that treatment with T-3 reduced the content o f phosphatidylinositol and increased the content of cardiolipin in the nucl ear fraction. In the liver homogenate, the content of phosphatidylinositol decreased and the content of phosphatidylethanolamine and cardiolipin incre ased after treatment with T-3. The total content of phospholipids after tre atment with T-3 remained unchanged, both in the nuclear fraction and in the liver homogenate. T-3 reduced the specific activity of phosphatidylcholine , phosphatidylserine, phosphatidylethanolamine and cardiolipin and had no e ffect on the specific activity of sphingomyelin and phosphatidylinositol ba th in the fraction of the nuclei and the liver homogenate. It is concluded that excess of triiodothyronine affects the content of phospholipids in the nuclei. The changes in the content of phospholipids in the nuclei largely reflect changes in their content in the liver.