The Polo-like kinase PLK-1 is required for nuclear envelope breakdown and the completion of meiosis in Caenorhabditis elegans

Citation
D. Chase et al., The Polo-like kinase PLK-1 is required for nuclear envelope breakdown and the completion of meiosis in Caenorhabditis elegans, GENESIS, 26(1), 2000, pp. 26-41
Citations number
49
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell & Developmental Biology
Journal title
GENESIS
ISSN journal
1526-954X → ACNP
Volume
26
Issue
1
Year of publication
2000
Pages
26 - 41
Database
ISI
SICI code
1526-954X(200001)26:1<26:TPKPIR>2.0.ZU;2-K
Abstract
The Polo-like kinases are key regulatory molecules required during the cell cycle for the successful completion of mitosis, We have cloned a C. elegan s homolog of the Drosophila melanogaster polo gene (designated plk-1 for C, elegans polo-like kinase-l) and present the subcellular localization of th e PLK-1 protein during the meiotic and mitotic cell cycles in C, elegans oo cytes and embryos, respectively. Disruption of PLK-1 expression by RNA-medi ated interference (RNAi) disrupts normal oocyte and embryonic development. Inspection of oocytes revealed a defect in nuclear envelope breakdown (NEBD ) before ovulation. This defect in NEED was also observed in oocytes that w ere depleted of the cyclin-dependent kinase NCC-1 (C. elegans homolog of Cd c2), The plk-1 RNAI oocytes were fertilized; however the resulting embryos were unable to separate their meiotic chromosomes or form and extrude polar bodies. These defects led to embryonic arrest as single cells. Published 2 000 Wiley-Liss, Inc.(dagger)