Natriuretic peptide system in the rat lacrimal gland

Citation
Es. Cho et al., Natriuretic peptide system in the rat lacrimal gland, EXP EYE RES, 71(4), 2000, pp. 333-340
Citations number
45
Language
INGLESE
art.tipo
Article
Categorie Soggetti
da verificare
Journal title
EXPERIMENTAL EYE RESEARCH
ISSN journal
0014-4835 → ACNP
Volume
71
Issue
4
Year of publication
2000
Pages
333 - 340
Database
ISI
SICI code
0014-4835(200010)71:4<333:NPSITR>2.0.ZU;2-H
Abstract
The presence and characteristics of the natriuretic peptides and their rece ptors in the rat exorbital lacrimal gland were investigated. Serial dilutio n curves of the grand extracts were parallel to the standard curves of synt hetic atrial natriuretic peptide (ANP) or C-type natriuretic peptide (CNP). Immunoreactive ANP or CNP in the gland extracts co-eluted with authentic A NP or CNP, and their contents were 4.95 +/- 0.60 and 2.87 +/- 0.53 pg mg(-1 ) protein (quadruplicate), respectively. By immunohistochemistry, strong im munoreactivities of ANP and CNP were co-localized in the tubules and excret ory ducts of the gland, and moderate immunoreactivities were found in the m yoepithelial cells and acini. Productions of guanosine 3',5'-cyclic monopho sphate by particulate guanylyl cyclase in the gland membranes were stimulat ed by natriuretic peptides in a dose-dependent manner, and that by CNP was larger than by ANP. Messenger RNAs for ANP, CNP and their receptors were de tected by reverse transcription-polymerase chain reaction. These results in dicate that natriuretic peptides and their specific receptors are found in the rat lacrimal gland. Therefore, it is suggested that natriuretic peptide system may play physiological roles in the rat lacrimal gland. (C) 2000 Ac ademic Press.