Induction of lef1 during zebrafish fin regeneration

Citation
Kd. Poss et al., Induction of lef1 during zebrafish fin regeneration, DEV DYNAM, 219(2), 2000, pp. 282-286
Citations number
19
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell & Developmental Biology
Journal title
DEVELOPMENTAL DYNAMICS
ISSN journal
1058-8388 → ACNP
Volume
219
Issue
2
Year of publication
2000
Pages
282 - 286
Database
ISI
SICI code
1058-8388(200010)219:2<282:IOLDZF>2.0.ZU;2-5
Abstract
Because the transcription factor Lef1 is important for development of sever al vertebrate organs but has not been investigated for involvement in epimo rphic regeneration, we examined its expression during regeneration of amput ated adult zebrafish caudal fins. We found that lef1 is markedly up-regulat ed in the newly formed wound epidermis of the fin regenerate and is maintai ned in the basal epidermal layer during formation of the regeneration blast ema, During regenerative outgrowth, lef1 expression is strongest in epiderm al cells adjacent to newly aligned scleroblasts that secrete bone matrix, w hile it is low or undetectable in epidermis adjacent to mesenchymal areas w ith either mature bone or proliferative distal blastema cells. This localiz ation is similar to that of the putative fin ray patterning signal Shh, In addition, brief treatments of fin regenerates with retinoic acid or the syn thetic Fgfr1 inhibitor SU5402 down-regulate epidermal lef1, similar to thei r effects on shh, These results suggest a role for Lef1 in scleroblast alig nment analogous to that proposed for Shh, Other Wnt signaling pathway membe rs wnt3 alpha, wnt5, and beta-catenin are also expressed in the fin regener ate. Our data suggest that Lef1 has specific roles in inducing and patterni ng vertebrate regenerating tissue. (C) 2000 Wiley-Liss, Inc.