A new way of achieving plant regeneration of Solanum lycopersicoides Dun. from root primordia culture

Citation
A. Tylicki et al., A new way of achieving plant regeneration of Solanum lycopersicoides Dun. from root primordia culture, PL CELL REP, 19(9), 2000, pp. 837-844
Citations number
34
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT CELL REPORTS
ISSN journal
0721-7714 → ACNP
Volume
19
Issue
9
Year of publication
2000
Pages
837 - 844
Database
ISI
SICI code
0721-7714(200009)19:9<837:ANWOAP>2.0.ZU;2-W
Abstract
Cell aggregates with root primordia were formed in root primordia culture ( RPC) of Solanum lycopersicoides grown in modified liquid MS medium containi ng 15 mg/l NAA. After transfer to liquid medium containing 1 mg/l 2,4-D, th e aggregates dissociated into single root primordia (RP) which had an organ izing root meristem at the apical pole. Oval structures called pseudoembryo s were formed from single RP. After passage to liquid MS medium without phy to-hormones and organic compounds (with the exception of sucrose), an apica l root meristem developed and the shoot apical meristem was initiated. The pseudoembryos developed into elongated pseudoseedlings which formed plants after transfer to a 1/2MSV medium. The development of pseudoembryos occurre d without the callus phase. Moreover, the induction of the shoot meristem o ccurred without exogenous cytokinins.