Superiority of mild hypothermic (20 degrees C) preservation for pancreaticmicrovasculature using the two-layer storage method

Citation
I. Matsumoto et al., Superiority of mild hypothermic (20 degrees C) preservation for pancreaticmicrovasculature using the two-layer storage method, PANCREAS, 21(3), 2000, pp. 305-309
Citations number
23
Language
INGLESE
art.tipo
Article
Categorie Soggetti
da verificare
Journal title
PANCREAS
ISSN journal
0885-3177 → ACNP
Volume
21
Issue
3
Year of publication
2000
Pages
305 - 309
Database
ISI
SICI code
0885-3177(200010)21:3<305:SOMH(D>2.0.ZU;2-7
Abstract
Hypothermia causes vascular endothelial damage that leads to graft microcir culation disorder and eventually thrombosis after reperfusion. The two-laye r cold storage method (TL) was previously demonstrated to supply oxygen to the pancreas graft and maintain high adenosine triphosphate tissue concentr ation. In this study, we evaluated whether mild hypothermic (20 degrees C) preservation using the TL method could reduce endothelial damage while main taining parenchymal viability. Graft survival by 20 degrees C preservation was investigated using a dog segmental pancreas autotransplantation model ( simple storage in University of Wisconsin solution (UW) for 5 and 8 hours o r TL for 5, 8, 12, and 24 hrs, respectively). Subsequently, the grafts were preserved in four different conditions (4 and 20 degrees C UW, 4 and 20 de grees C TL) for 8 hours to evaluate microvascular endothelial damage. Trypa n blue uptake of vascular endothelium and pancreatic tissue perfusion were evaluated. No graft preserved by 20 degrees C UW for 5 and 8 hours survived (0/7 and 0/4). In contrast, the graft survival rates by 20 degrees C TL fo r 5, 8, 12, and 24 hours were 100% (5/5), 80% (4/5), 20% (1/5), and 0% (0/4 ), respectively. In trypan blue uptake analysis, there were significant dif ferences between 4 and 20 degrees C in both UW and TL (4 degrees C UW, 37% [n = 5] vs. 20 degrees C UW, 13% [n = 4] [p < 0.01], 4 degrees C TL, 29% [n = 5] vs. 20 degrees C TL, 10% [n = 5] [p < 0.01]). The perfusion values in 20 degrees C TL were significantly higher than those in other groups at le ast for up to 120 minutes after reperfusion (p < 0.01). In short-term pancr eas preservation, mild hypothermic TL reduced vascular endothelial cell dam age and ameliorated graft microcirculation while maintaining parenchymal vi ability. Mild hypothermic TL may lessen vascular complications in clinical pancreas transplantation when used for several-hour preservation.