Development of a rapid and sensitive bioassay device using human cells immobilized in macroporous microcarriers for the on-site evaluation of environmental waters

Citation
R. Shoji et al., Development of a rapid and sensitive bioassay device using human cells immobilized in macroporous microcarriers for the on-site evaluation of environmental waters, APPL MICR B, 54(3), 2000, pp. 432-438
Citations number
9
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
ISSN journal
0175-7598 → ACNP
Volume
54
Issue
3
Year of publication
2000
Pages
432 - 438
Database
ISI
SICI code
0175-7598(200009)54:3<432:DOARAS>2.0.ZU;2-D
Abstract
We developed a novel disposable bioassay device based on the fluorescein is othiocyanate-labelled low-density lipoprotein-uptake activity of human hepa toblastoma Hep G2 cells. The cells were cultured in porous microcarriers at a high cell density and packed in a filter lip that has a hydrophobic memb rane. Upon evaluation of water samples, the culture medium was decanted by pipetting it down with a micropipet, and the samples were then introduced t o the cell-immobilizing part of the tip only by pipetting them up after mix ing them with x10 concentrated culture medium. The new device enabled us to detect almost the same toxicity levels of river water within 2 h of exposu re as those detected by a conventional 48-h cell-survival assay. This is th e first bioassay device for the rapid on-site evaluation of environmental w aters using cultured human cells, and therefore promising for water-quality management based on risk to humans.