Dual transcriptional regulation of the Escherichia coli phosphate-starvation-inducible psiE gene of the phosphate regulon by PhoB and the cyclic AMP (cAMP)-cAMP receptor protein complex

Citation
Sk. Kim et al., Dual transcriptional regulation of the Escherichia coli phosphate-starvation-inducible psiE gene of the phosphate regulon by PhoB and the cyclic AMP (cAMP)-cAMP receptor protein complex, J BACT, 182(19), 2000, pp. 5596-5599
Citations number
27
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF BACTERIOLOGY
ISSN journal
0021-9193 → ACNP
Volume
182
Issue
19
Year of publication
2000
Pages
5596 - 5599
Database
ISI
SICI code
0021-9193(200010)182:19<5596:DTROTE>2.0.ZU;2-B
Abstract
We have shown that the Escherichia coli phosphate-starvation-inducible psiE gene is regulated by both phosphate and the carbon source by using both la cZ and chloramphenicol acetyltransferase gene (cat) fusions. Yet, under all conditions tested, a single transcriptional start site lying 7 bp downstre am of a predicted -10 region was revealed by primer extension analysis, DNa se I footprinting showed that the PhoB transcriptional-activator protein pr otects two predicted pho buses lying upstream of and near the -35 promoter region. Similar analysis showed that the cyclic AMP (cAMP)-cAMP receptor pr otein (cAMP-CRP) complex binds a region that overlaps with the downstream p ho box. These results, together with measurements of the in vivo psiE promo ter activity under various conditions, show that expression of the psiE gen e is under direct positive and negative control by PhoB and cAMP CRP, respe ctively.