Imprinting of human GRB10 and its mutations in two patients with Russell-Silver syndrome

Citation
H. Yoshihashi et al., Imprinting of human GRB10 and its mutations in two patients with Russell-Silver syndrome, AM J HU GEN, 67(2), 2000, pp. 476-482
Citations number
51
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology","Molecular Biology & Genetics
Journal title
AMERICAN JOURNAL OF HUMAN GENETICS
ISSN journal
0002-9297 → ACNP
Volume
67
Issue
2
Year of publication
2000
Pages
476 - 482
Database
ISI
SICI code
0002-9297(200008)67:2<476:IOHGAI>2.0.ZU;2-Q
Abstract
Documentation of maternal uniparental disomy of chromosome 7 in 10% of pati ents with Russell-Silver syndrome (RSS), characterized by prenatal and post natal growth retardation and dysmorphic features, has suggested the presenc e of an imprinted gene on chromosome 7 whose mutation is responsible for th e RSS phenotype, Human GRB10 on chromosome 7, a homologue of the mouse impr inted gene Grb10, is a candidate, because GRB10 has a suppressive effect on growth, through its interaction with either the IGF-I receptor or the GH r eceptor, and two patients with RSS were shown to have a maternally derived duplication of 7p11-p13, encompassing GRB10. In the present study, we first demonstrated that the GRB10 gene is also monoallelically expressed in huma n fetal brain tissues and is transcribed from the maternally derived allele in somatic-cell hybrids. Hence, human GRB10 is imprinted. A mutation analy sis of GRB10 in 58 unrelated patients with RSS identified, within the N-ter minal domain of the protein, a P95S substitution in two patients with RSS. In these two cases, the mutant allele was inherited from the mother. The fa ct that monoallelic GRB10 expression was observed from the maternal allele in this study suggests but does not prove that these maternally transmitted mutant alleles contribute to the RSS phenotype.